NCMIR Pioneers Use of Eosin for Fluorescence Photooxidation as a Staining Technique
A projection of a series of optical sections through a Purkinje neuron reveals both the overall morphology (red) and the dendritic spines (green). The unconjugated red dye fills the entire dendritic tree when injected, including shafts and spines. In contrast, the phalloidin-Alexa 488 conjugate selectively labels the spines. Image by Francisco Capani and Eric Bushong.